Summary
Type: SPE
Application #: 300380
Catalog#: 730014 - SPE Cartridges, Chromabond, C18 ec, 6ml, PP, 500mg, 30/pk
Detection:
Conditions: Conditioning of the 1st column:
10 ml methanol, then 10 ml methanol / water (4 : 1, v/v)
Sample application:
Slowly force or aspirate the centrifuged solution through the column; dilute
the eluate with twice its volume of dist. water
Conditioning of the 2nd column:
10 ml methanol, then 10 ml dist. water
Sample application:
Slowly force or aspirate the diluted eluate from the 1st column through the 2nd
column
Column washing:
5 ml dist. water, dry column by aspirating air through it; then rinse column
with 5 ml n-hexane, dry under vacuum for 3 – 4 min
Elution:
Elute B1 with 3 ml n-hexane / dichloromethane (45 : 55, v/v); dry column 3 – 4
min under vacuum and elute M1 with 3 ml dichloromethane / acetone (9 : 1, v/v)
Authors: LC department
Source: Macherey-Nagel